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Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with <t>NBD</t> <t>C6-ceramide</t> (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.
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Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with <t>NBD</t> <t>C6-ceramide</t> (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.
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Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with <t>NBD</t> <t>C6-ceramide</t> (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.
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Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with <t>NBD</t> <t>C6-ceramide</t> (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.
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Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with <t>NBD</t> <t>C6-ceramide</t> (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.
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Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with <t>NBD</t> <t>C6-ceramide</t> (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.
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Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with <t>NBD</t> <t>C6-ceramide</t> (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.
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Image Search Results


Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with NBD C6-ceramide (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.

Journal: Microorganisms

Article Title: EhVps29 Has a Role in the Location of the Retromer Complex and the Function of Key Virulence Factors in Entamoeba histolytica

doi: 10.3390/microorganisms14050976

Figure Lengend Snippet: Localization of EhVps29 and EhVps26 in Golgi-like structures in trophozoites. ( A ) Confocal microscopy of control and knocked-down cells incubated with NBD C6-ceramide (green), α-EhVps29 (blue) and α-EhVps26 (red) antibodies. Zoom images: Marked squares in ( a , b ). Arrows: Co-localization areas of EhVps29 and EhVps26 proteins in the Golgi-like structure. ( B ) Pearson’s correlation between the Golgi marker and EhVps29 or EhVps26. Plot is representative of twenty 0.5 µm z-stack images from control and knockdown cells. ** p < 0.01, *** p < 0.001.

Article Snippet: We used the NBD C6 ceramide probe (ThermoFisher, Waltham, MA, USA) as a Golgi apparatus marker, according to the manufacturer’s guide.

Techniques: Confocal Microscopy, Control, Incubation, Marker, Knockdown